High Primer Concentration Improves PCR Amplification from Random Pools
نویسندگان
چکیده
منابع مشابه
High primer concentration improves PCR amplification from random pools.
The success of PCR is partly based on its exponential amplification characteristics. Nevertheless, in practice, the achievable yields are limited. Figure 1A and B shows the quantification of a typical reaction with varying but defined amounts of starting template. Every five cycles an aliquot of the reaction was removed and afterwards the amount of accumulated product quantitated. Although, ini...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.5.985